Inexpensive Rain Gauges Constructed from Recyclable 2-Liter Plastic Soft Drink Bottles

A less laborious approach to the high-throughput production of recombinant proteins in Escherichia coli using 2-liter plastic bottles.

Contemporary approaches to biology often call for the high-throughput production of large amounts of numerous proteins for structural or functional studies. Even with the highly efficient protein expression systems developed in Escherichia coli, production of these proteins is laborious and time-consuming. We have simplified established protocols by the use of disposable culture vessels: common...

Sorting Plastic Bottles for Recycling

Scrambling Eggs in Plastic Bottles

This issue of PLoS Genetics contains the second of two important papers describing the high levels of meiotic failure caused by exposure of female mice to a chemical known as Bisphenol A (BPA) [1]. In the first article [2], Pat Hunt and her collaborators demonstrated high levels of meiotic failure in females exposed to BPA, suggesting that BPA exposure affects maturing oocytes. This conclusion ...

Arthroscopic removal of a plastic soft drink bottle cap in the knee: a case report

We report a rare case of late knee locking after an open knee injury in a polytrauma patient with a pelvic fracture and a contralateral femoral artery injury. Once the life and limb threatening injuries were addressed, debridement and washout of the knee wound was performed. X-rays and subsequent CT revealed only an undisplaced patella fracture. The patient presented 6 months later to a knee su...

Uncertainty in the Spatial Interpolation of Urban Rain Gauges Data

Rainfall data is useful in many fields such as urban management, agriculture, and so on. Spatial interpolation is widely used to interpolation continue rainfall data from discrete rainfall gauges. The uncertainty in spatial interpolation is change in different region. Paper focus on urban small area of Beijing city, Xicheng District and analyses uncertainty of spatial interpolation from four as...